Journal article

Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier

Ingrid Kratzer, Alexandre Vasiljevic, Catherine Rey, Michelle Fevre-Montange, Norman Saunders, Nathalie Strazielle, Jean-Francois Ghersi-Egea



The choroid plexus epithelium controls the movement of solutes between the blood and the cerebrospinal fluid. It has been considered as a functionally more immature interface during brain development than in adult. The anatomical basis of this barrier is the interepithelial choroidal junction whose tightness has been attributed to the presence of claudins. We used quantitative real-time polymerase chain reaction, Western blot and immunohistochemistry to identify different claudins in the choroid plexuses of developing and adult rats. Claudin-1, -2, and -3 were highly and selectively expressed in the choroid plexus as compared to brain or parenchyma microvessels and were localized at epitheli..

View full abstract


Awarded by European Union Seventh Framework Program

Funding Acknowledgements

The research leading to these results has received funding from the European Union Seventh Framework Program (FP7/2007-2013) under grant agreement no HEALTH-F2-2009-241778. We thank Alan S. L. Yu from the Department of Medicine, Division of Nephrology, University of Southern California Keck School of Medicine, Los Angeles, California, who kindly provided the anti-Cld 19 antibody for these studies. We thank Chantal Watrin for her excellent technical help.