Journal article

Uterine Lavage or Aspirate: Which View of the Intrauterine Environment?

NJ Hannan, G Nie, A Rainzcuk, LJF Rombauts, LA Salamonsen

Reproductive Sciences | SAGE PUBLICATIONS INC | Published : 2012

Abstract

Fluid within the uterine cavity provides the microenvironment for preimplantation blastocyst development and early implantation. Analysis of uterine fluid sampled by aspiration or lavage provides a view of this microenvironment but the similarity or otherwise of the sample components is not known. This study compared proteins in aspirates versus lavage samples taken sequentially from the same women, using surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS), multiplex cytokine assays, and an activity assay for proprotein convertase 6. Both lavage and aspiration enabled analysis of uterine fluid components, but they provided substantially different prot..

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Grants

Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

The author(s) disclosed receipt of the following financial support for the research, authorship and/or publication of this article: supported by the National Health and Medical Research Council of Australia, Program grant #494802, Project grant #611804 (GN & LJFR) and Fellowships #1002028 (LAS) #494808 (GN) #628927 (NH); by Monash IVF and by the Victorian Government's Operational Infrastructure Support Program.