Journal article

Maturation of the Mitochondrial Redox Response to Profound Asphyxia in Fetal Sheep

Paul P Drury, Laura Bennet, Lindsea C Booth, Joanne O Davidson, Guido Wassink, Alistair J Gunn

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2012

Abstract

UNLABELLED: Fetal susceptibility to hypoxic brain injury increases over the last third of gestation. This study examined the hypothesis that this is associated with impaired mitochondrial adaptation, as measured by more rapid oxidation of cytochrome oxidase (CytOx) during profound asphyxia. METHODS: Chronically instrumented fetal sheep at 0.6, 0.7, and 0.85 gestation were subjected to either 30 min (0.6 gestational age (ga), n = 6), 25 min (0.7 ga, n = 27) or 15 min (0.85 ga, n = 17) of complete umbilical cord occlusion. Fetal EEG, cerebral impedance (to measure brain swelling) and near-infrared spectroscopy-derived intra-cerebral oxygenation (ΔHb = HbO(2) - Hb), total hemoglobin (THb) and C..

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Grants

Awarded by National Institutes of Health


Awarded by NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE


Funding Acknowledgements

This study was supported by the Health Research Council of New Zealand, the Auckland Medical Research Foundation and National Institutes of Health grant R21 NS063141-01A1. P Drury is supported by the New Zealand Neurological Foundation W&B Miller Doctoral Scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.