A Protocol for Measurement of Noncoding RNA in Human Serum
Caroline J Taylor, Sarang N Satoor, Amaresh K Ranjan, Maria V Pereira e Cotta, Mugdha V Joglekar
EXPERIMENTAL DIABETES RESEARCH | HINDAWI PUBLISHING CORPORATION | Published : 2012
MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs. Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease. Currently, there are many methods of isolating total RNA from liquid samples. Here, we describe a simple, cost effective method for extraction of RNA from human serum as well as subsequent real time PCR analysis of miRNA levels.