Modification of the size distribution of lysozyme microbubbles using a post-sonication technique

Abstract

The control of the physical and functional properties of ultrasound responsive microbubbles is of interest because of their potential applications in therapeutic and diagnostic medicine. Crosslinked lysozyme microbubbles, synthesized using high intensity low frequency (20kHz) ultrasound, possess long-term stability and retain bactericidal property. However, the relatively broader size distribution of these microbubbles may limit their use in some applications. In this article, we introduce a post-sonication technique for modifying the size distribution of lysozyme microbubbles. The post-sonication of these microbubbles at very high ultrasound frequencies (213, 355, 647, and 1056kHz) led to t..