Transcriptional gene silencing of HIV-1 through promoter targeted RNA is highly specific
Kazuo Suzuki, Takaomi Ishida, Makoto Yamagishi, Chantelle Ahlenstiel, Sanjay Swaminathan, Katharine Marks, Daniel Murray, Erin M McCartney, Michael R Beard, Marina Alexander, Damian FJ Purcell, David A Cooper, Toshiki Watanabe, Anthony D Kelleher
RNA BIOLOGY | TAYLOR & FRANCIS INC | Published : 2011
We have previously reported induction of transcriptional gene silencing (TGS) of HIV-1 by short hairpin RNA (shRNA) expressed in MOLT-4 cells. The shRNA (termed shPromA) targets the highly conserved tandem NF-kB binding sequences of the HIV-1 promoter. Recent articles have reported that TGS mediated by promoter-targeted siRNAs was exclusively the result of sequence non-specific off-target effects. Specifically, several mismatched siRNAs to the target promoter sequences were reported to also induce significant TGS, suggesting TGS was a consequence of off-target effects. Here, following extensive investigation, we report that shPromA induces sequence specific transcriptional silencing in HIV-1..View full abstract
This paper was funded from the following sources: the Australian Government Department of Health and Ageing; grants from the National Health and Medical Research Council including a Practitioner Fellowship (A.K.) and a Project grant (K.S., C.H.). The views expressed in this publication do not necessarily represent the position of the Australian Government. The Kirby Institute is affiliated with the Faculty of Medicine, University of New South Wales.