Journal article

Transcriptional gene silencing of HIV-1 through promoter targeted RNA is highly specific

Kazuo Suzuki, Takaomi Ishida, Makoto Yamagishi, Chantelle Ahlenstiel, Sanjay Swaminathan, Katharine Marks, Daniel Murray, Erin M McCartney, Michael R Beard, Marina Alexander, Damian FJ Purcell, David A Cooper, Toshiki Watanabe, Anthony D Kelleher

RNA BIOLOGY | TAYLOR & FRANCIS INC | Published : 2011

Abstract

We have previously reported induction of transcriptional gene silencing (TGS) of HIV-1 by short hairpin RNA (shRNA) expressed in MOLT-4 cells. The shRNA (termed shPromA) targets the highly conserved tandem NF-kB binding sequences of the HIV-1 promoter. Recent articles have reported that TGS mediated by promoter-targeted siRNAs was exclusively the result of sequence non-specific off-target effects. Specifically, several mismatched siRNAs to the target promoter sequences were reported to also induce significant TGS, suggesting TGS was a consequence of off-target effects. Here, following extensive investigation, we report that shPromA induces sequence specific transcriptional silencing in HIV-1..

View full abstract

University of Melbourne Researchers