The Outcome of Renal Ischemia-Reperfusion Injury Is Unchanged in AMPK-beta 1 Deficient Mice
Peter F Mount, Kurt Gleich, Shanna Tam, Scott A Fraser, Suet-Wan Choy, Karen M Dwyer, Bo Lu, Bryce Van denderen, Guenter Fingerle-Rowson, Richard Bucala, Bruce E Kemp, David A Power
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2012
AIM: Activation of the master energy-regulator AMP-activated protein kinase (AMPK) in the heart reduces the severity of ischemia-reperfusion injury (IRI) but the role of AMPK in renal IRI is not known. The aim of this study was to determine whether activation of AMPK by acute renal ischemia influences the severity of renal IRI. METHODS: AMPK expression and activation and the severity of renal IRI was studied in mice lacking the AMPK β1 subunit and compared to wild type (WT) mice. RESULTS: Basal expression of activated AMPK, phosphorylayed at αThr¹⁷², was markedly reduced by 96% in AMPK-β1⁻/⁻ mice. Acute renal ischaemia caused a 3.2-fold increase in α1-AMPK activity and a 2.5-fold increase in..View full abstract
This study was performed with funding from the Australian National Health and Medical Research Council (NHMRC) (www.nhmrc.gov.au) and the Austin Hospital Medical Research Foundation (AHMRF) (www.ahmrf.org.au). The funders had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript.