Journal article
Survival of rat functional dental pulp cells in vascularized tissue engineering chambers
T Srisuwan, DJ Tilkorn, S Al-Benna, A Vashi, A Penington, HH Messer, KM Abberton, EW Thompson
Tissue and Cell | Published : 2012
Abstract
Regenerative endodontics aims to preserve, repair or regenerate the dental pulp tissue. Dental pulp stem cells, have a potential use in dental tissue generation. However, specific requirements to drive the dental tissue generation are still obscured. We established an in vivo model for studying the survival of dental pulp cells (DPC) and their potential to generate dental pulp tissue. DPC were mixed with collagen scaffold with or without slow release bone morphogenic protein 4 (BMP-4) and fibroblast growth factor 2 (FGF2). The cell suspension was transplanted into a vascularized tissue engineering chamber in the rat groin. Tissue constructs were harvested after 2, 4, 6, and 8 weeks and proce..
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Funding Acknowledgements
This investigation was supported in part by Australian Dental Research Foundation and Australian Society of Endodontology, Australia. We gratefully acknowledge the morphometric guidance of Dr. Geraldine Mitchell, the technical guidance of Mrs. Effie Keramidaris and Mr. Jason Palmer, and the overall support of Professor Wayne Morrison, from the Bernard O'Brien Institute of Medical Research.