Journal article

Structural Basis of Rotavirus Strain Preference toward N-Acetyl- or N-Glycolylneuraminic Acid-Containing Receptors

Xing Yu, Vi T Dang, Fiona E Fleming, Mark von Itzstein, Barbara S Coulson, Helen Blanchard

JOURNAL OF VIROLOGY | AMER SOC MICROBIOLOGY | Published : 2012

Abstract

The rotavirus spike protein domain VP8* is essential for recognition of cell surface carbohydrate receptors, notably those incorporating N-acylneuraminic acids (members of the sialic acid family). N-Acetylneuraminic acids occur naturally in both animals and humans, whereas N-glycolylneuraminic acids are acquired only through dietary uptake in normal human tissues. The preference of animal rotaviruses for these natural N-acylneuraminic acids has not been comprehensively established, and detailed structural information regarding the interactions of different rotaviruses with N-glycolylneuraminic acids is lacking. In this study, distinct specificities of VP8* for N-acetyl- and N-glycolylneurami..

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University of Melbourne Researchers

Grants

Awarded by Australian Research Council (ARC)


Awarded by National Health and Medical Research Council of Australia (NHMRC)


Awarded by NHMRC


Funding Acknowledgements

H.B. acknowledges financial support for access to synchrotron facilities under the Access to Major Research Facilities Program within the International Science Linkages Program of the Australian Government. We thank staff at beam line 8.3.1 at the Advanced Light Source (ALS) for assistance in data collection. Beam line 8.3.1 at the Lawrence Berkeley National Laboratory, ALS was funded by the NSF, the University of California and Henry Wheeler. This work was supported by the Australian Research Council (ARC; DP0774383) and the National Health and Medical Research Council of Australia (NHMRC; ID597439). B. S. C. is a Senior Research Fellow (ID 628319) of the NHMRC. M. V. I thanks the ARC for the award of an Australian Federation Fellowship.