Electrophoretic mobilities of proteins and protein mixtures in porous membranes

Abstract

The electrophoresis of bovine serum albumin (BSA) and bovine haemoglobin (BHb) through microfiltration membranes has been investigated using an apparatus consisting of two well-stirred chambers separated by a microfiltration membrane, with electrode compartments at each end. The electrophoretic mobilities of the proteins were determined under conditions of zero concentration driving force and with negligible adsorption on to the membranes occurring during an experiment. Using a track-etched membrane of 9% porosity and 0.2 μm pore size, the initial mobilities of both proteins were close to published free-solution values at the same pH, after correction for the ionic strength of the buffer, th..