Journal article
Caveolin-1 orchestrates the balance between glucose and lipid-dependent energy metabolism: Implications for liver regeneration
M Alejandro Fernández-Rojo, C Restall, C Ferguson, N Martel, S Martin, M Bosch, A Kassan, GM Leong, SD Martin, SL Mcgee, GEO Muscat, RL Anderson, C Enrich, A Pol, RG Parton
Hepatology | WILEY | Published : 2012
DOI: 10.1002/hep.24810
Abstract
Caveolin-1 (CAV1) is a structural protein of caveolae involved in lipid homeostasis and endocytosis. Using newly generated pure Balb/C CAV1 null (Balb/CCAV1-/-) mice, CAV1-/- mice from Jackson Laboratories (JAXCAV1-/-), and CAV1-/- mice developed in the Kurzchalia Laboratory (KCAV1-/-), we show that under physiological conditions CAV1 expression in mouse tissues is necessary to guarantee an efficient progression of liver regeneration and mouse survival after partial hepatectomy. Absence of CAV1 in mouse tissues is compensated by the development of a carbohydrate-dependent anabolic adaptation. These results were supported by extracellular flux analysis of cellular glycolytic metabolism in CAV..
View full abstractGrants
Awarded by Spanish Ministerio de Ciencia e Innovacion
Awarded by Fundacio Marato TV3
Funding Acknowledgements
Supported by grants from the National Health and Medical Research Council of Australia (to R. G. P., R. L. A.) and from the National Breast Cancer Foundation (R. L. A.). M.A.F.-R. was supported by the Program of MEC/Fulbright postdoctoral fellowships from Ministry of Education and Science from the Spanish Government. A. P. is supported by grants (BFU2008-00345/BMC and CSD2009-00016PI040820) and C. E. (BFU2009-10335, CSD2009-00016) from the Spanish Ministerio de Ciencia e Innovacion and PI040236 (Fundacio Marato TV3). G. M. L. is supported by an NHMRC Clinical Career Development Award.We are grateful to the Australian Cancer Research Foundation (ACRF)/Institute for Molecular Bioscience (IMB) Dynamic Imaging Facility for Cancer Biology, established with funding from the ACRF. The authors acknowledge the use of the Australian Microscopy and Microanalysis Facility at the Center for Microscopy and Microanalysis at The University of Queensland. We thank Lukas Bahati and James Rae for assistant in lipid extraction and TLC performance, and Brian Bynon and Mark Ropper from the Clinical Pathology Laboratory at the University of Queensland for their assistance in the analysis of mouse plasma.