Journal article

Use of a phage-based assay for phenotypic detection of mycobacteria directly from sputum

DJ Park, FA Drobniewski, A Meyer, SM Wilson

Journal of Clinical Microbiology | AMER SOC MICROBIOLOGY | Published : 2003

Abstract

The phage amplified biologically assay is a new method for rapid and low-cost phenotypic determination of the drug sensitivities of Mycobacterium tuberculosis isolates and the detection of viable organisms in patient specimens. Infection of slowly growing mycobacteria with phage (phage D29) was followed by chemical virucide destruction of extracellular phage. Infected mycobacteria were mixed in culture with rapidly growing sensor cells, which the phage can also infect; i.e., lytic amplification of phage occurs. The aims of the present study were to optimize the speed and sensitivity of the assay and reduce its cost for developing countries by using an M. tuberculosis-spiked sputum model with..

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