Fructose diet treatment in mice induces fundamental disturbance of cardiomyocyte Ca2 handling and myofilament responsiveness
Kimberley M Mellor, Igor R Wendt, Rebecca H Ritchie, Lea MD Delbridge
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | AMER PHYSIOLOGICAL SOC | Published : 2012
High fructose intake has been linked to insulin resistance and cardiac pathology. Dietary fructose-induced myocardial signaling and morphological alterations have been described, but whether cardiomyocyte function is influenced by chronic high fructose intake is yet to be elucidated. The goal of this study was to evaluate the cardiomyocyte excitation-contraction coupling effects of high dietary fructose and determine the capacity for murine cardiomyocyte fructose transport. Male C57Bl/6J mice were fed a high fructose diet for 12 wk. Fructose- and control-fed mouse cardiomyocytes were isolated and loaded with the fura 2 Ca(2+) fluorescent dye for analysis of twitch and Ca(2+) transient charac..View full abstract
The authors acknowledge funding support from the Diabetes Australia Research Trust and the National Heart Foundation of Australia. R. H. Ritchie is supported by a National Health and Medical Research Council of Australia Senior Research Fellowship.