Journal article
Aggrecanase cleavage in juvenile idiopathic arthritis patients is minimally detected in the aggrecan interglobular domain but robust at the aggrecan C-terminus
A Struglics, LS Lohmander, K Last, J Akikusa, R Allen, AJ Fosang
Arthritis and Rheumatism | WILEY-BLACKWELL | Published : 2012
DOI: 10.1002/art.34665
Abstract
Objective To investigate aggrecan degradation in juvenile idiopathic arthritis (JIA). Methods The pattern and abundance of aggrecan fragments in synovial fluid (SF) aspirates from JIA patients were analyzed and compared with aggrecan fragments in SF from patients with other arthritides, children with knee injury, and a knee-healthy reference group. Concentrations of sulfated glycosaminoglycan (sGAG) in SF were measured by Alcian blue precipitation assay. Aggrecan fragments were purified by dissociative CsCl density-gradient centrifugation, deglycosylated, and analyzed by Western blot using antibodies specific for either aggrecanase-derived ARGS, SELE, and KEEE neoepitopes or the aggrecan G3 ..
View full abstractGrants
Funding Acknowledgements
Supported by a Solander Traveling Grant and a Maggie Stephens Travel Grant (to Dr. Struglics) and by grants from the Lynne Quayle Charitable Trust and Arthritis Australia (to Dr. Fosang). Dr. Struglics' work was also supported by the Crafoord Foundation, the Lund University Medical Faculty, King Gustaf V's 80-Year Fund, the Swedish Rheumatism Association, and the A. Osterlunds Foundation. Dr. Lohmander's work was supported by King Gustaf V's 80-Year Fund, the Swedish Rheumatism Association, and the Swedish Research Council. Ms. Last and Dr. Fosang's work was also supported by the National Health and Medical Research Council (Australia) and the Victorian Government's Operational Infrastructure Support Program.