Journal article
TRPV1 marks synaptic segregation of multiple convergent afferents at the rat medial solitary tract nucleus
JH Peters, SJ McDougall, JA Fawley, MC Andresen
Plos One | Published : 2011
Abstract
TRPV1 receptors are expressed on most but not all central terminals of cranial visceral afferents in the caudal solitary tract nucleus (NTS). TRPV1 is associated with unmyelinated C-fiber afferents. Both TRPV1+ and TRPV1- afferents enter NTS but their precise organization remains poorly understood. In horizontal brainstem slices, we activated solitary tract (ST) afferents and recorded ST-evoked glutamatergic excitatory synaptic currents (ST-EPSCs) under whole cell voltage clamp conditions from neurons of the medial subnucleus. Electrical shocks to the ST produced fixed latency EPSCs (jitter<200 μs) that identified direct ST afferent innervation. Graded increases in shock intensity often recr..
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Grants
Awarded by National Heart, Lung, and Blood Institute
Funding Acknowledgements
This work was supported by grants from the National Institutes of Health (NIH), Ruth L. Kirschstein Individual National Research Service Award HL-88894 (Dr. Peters), National Health and Medical Research Council of Australia, Overseas Training C J Martin Fellowship #400405 (Dr. McDougall), HL-41119 (Dr. Andresen), and HL-105703 (Dr. Andresen). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Heart, Lung and Blood Institute or the NIH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.