Journal article

Human and Mouse Type I Natural Killer T Cell Antigen Receptors Exhibit Different Fine Specificities for CD1d-Antigen Complex

Kwok S Wun, Fiona Ross, Onisha Patel, Gurdyal S Besra, Steven A Porcelli, Stewart K Richardson, Santosh Keshipeddy, Amy R Howell, Dale I Godfrey, Jamie Rossjohn

JOURNAL OF BIOLOGICAL CHEMISTRY | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2012

Abstract

Human and mouse type I natural killer T (NKT) cells respond to a variety of CD1d-restricted glycolipid antigens (Ags), with their NKT cell antigen receptors (NKT TCRs) exhibiting reciprocal cross-species reactivity that is underpinned by a conserved NKT TCR-CD1d-Ag docking mode. Within this common docking footprint, the NKT TCR recognizes, to varying degrees of affinity, a range of Ags. Presently, it is unclear whether the human NKT TCRs will mirror the generalities underpinning the fine specificity of the mouse NKT TCR-CD1d-Ag interaction. Here, we assessed human NKT TCR recognition against altered glycolipid ligands of α-galactosylceramide (α-GalCer) and have determined the structures of a..

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University of Melbourne Researchers

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Awarded by Direct For Mathematical & Physical Scien


Funding Acknowledgements

This research was supported by the National Health and Medical Research Council of Australia (NHMRC), the ARC, the Cancer Council of Victoria and an NIH RO1 Grant GM 087136.Supported by an ARC Future Fellowship.Supported by an NHMRC Senior Principal Research Fellowship. To whom correspondence may be addressed. Tel.: 613-8344-5689; Fax:613-93471540; E-mail: godfrey@unimelb.edu.au.Supported by an NHMRC Australia Fellowship. To whom correspondence may be addressed. Tel: 613-99029236; Fax: 613-99025000; E-mail: jamie.rossjohn@monash.edu.