Journal article

Intranasal immunization with yeast-expressed 19 kD carboxyl-terminal fragment of Plasmodium yoelii merozoite surface protein-1 (yMSP1(19)) induces protective immunity to blood stage malaria infection in mice

C Hirunpetcharat, D Stanisic, XQ Liu, J Vadolas, RA Strugnell, R Lee, LH Miller, DC Kaslow, MF Good

PARASITE IMMUNOLOGY | BLACKWELL SCIENCE LTD | Published : 1998

Abstract

Variable protection against malaria blood-stage infection has been demonstrated in mice following parenteral immunization with the highly conserved 19 kD carboxylterminal fragment of the merozoite surface protein-1 (MSP119) using CFA/IFA and other adjuvants. Here we show that intranasal immunization of BALB/C mice with yeast expressed Plasmodium yoelii MSP119 plus a mixture of native and recombinant cholera toxin B subunit, could induce serum MSP119-specific antibodies at titres ranging from 20 000 to 2 560 000. The Ig subclass responses were predominantly G1 and G2b. Intranasal immunization led to protection following challenge (peak parasitaemia /= 640 000). In two of the three protected m..

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