Divergent lymphocyte signalling revealed by a powerful new tool for analysis of time-lapse microscopy
Kim Pham, Raz Shimoni, Mandy J Ludford-Menting, Cameron J Nowell, Pavel Lobachevsky, Ze'ev Bomzon, Min Gu, Terence P Speed, C Jane McGlade, Sarah M Russell
Immunology and Cell Biology | NATURE PUBLISHING GROUP | Published : 2013
OP9-Dll1 cells were from Juan-Carlos Zuniga-Pflucker (University of Toronto, Canada), and the mCherry-tubulin expression construct was provided by Stephen Ting (Peter MacCallum Cancer Centre). Microfabricated cell paddocks were supplied by Daniel Day (Swinburne University and Microsurfaces Pty Ltd). We thank the MATLAB community for sharing many useful open codes. The work was funded by the Australian National Health and Medical Research Council (NHMRC), the Human Frontiers Science Programme, the Australian Research Council (ARC) and the Australian Cancer Research Foundation (ACRF), and supported by the animal, flow cytometry and microscopy core facilities of the PeterMac. We thank members of the Russell Lab, David Izon, Patrick Humbert, Ellen Robey, Sarah Ellis, Joe Trapani and Moshe Olshansky for helpful advice and discussions.