Journal article
c-Jun NH2-terminal kinase activity in subcutaneous adipose tissue but not nuclear factor-κB activity in peripheral blood mononuclear cells is an independent determinant of insulin resistance in healthy individuals
KC Sourris, JG Lyons, MPJ De Courten, SL Dougherty, DC Henstridge, ME Cooper, M Hage, A Dart, BA Kingwell, JM Forbes, B De Courten
Diabetes | AMER DIABETES ASSOC | Published : 2009
DOI: 10.2337/db08-1725
Abstract
OBJECTIVE - Chronic low-grade activation of the immune system (CLAIS) predicts type 2 diabetes via a decrease in insulin sensitivity. Our study investigated potential relationships between nuclear factor-κB (NF-κB) and c-Jun NH2-terminal kinase (JNK) pathways - two pathways proposed as the link between CLAIS and insulin resistance. RESEARCH DESIGN AND METHODS - Adiposity (dualenergy X-ray absorptiometry), waist-to-hip ratio (WHR), and insulin sensitivity (M, hyperinsulinemic-euglycemic clamp) were measured in 22 healthy nondiabetic volunteers (aged 29 ± 11 years, body fat 28 ± 11%). NF-κB activity (DNA-binding assay) and JNK1/2 activity (phosphorylated JNK) were assessed in biopsies of the v..
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Funding Acknowledgements
[ "This work was supported by the National Health and Medical Research Council of Australia (Project, Fellowship and Centre for Clinical Research Excellence grants). This work was also supported by the Bennelong Foundation and Pfizer Cardiovascular Lipid Research grants. No other potential conflicts of interest relevant to this article were reported. We thank all volunteers for their participation in the study.", "We are grateful for the medical supervision of Stephen Duffy and the technical expertise of Kirsten Howlett, Melissa Formosa, Dona Onan, Brian Drew, Anna Gasser, Alaina Natoli, and Sally Penfold." ]