Somatostatin receptors in wildtype and somatostatin deficient mice and their involvement in nitric oxide physiology in the retina

Abstract

The present study investigated the localization and density of somatostatin (SRIF) receptor subtypes (sst1-5) and SRIF-nitric oxide (NO{radical dot}) interactions in the retina of wildtype [WT, (+/+)] and somatostatin deficient mice [SRIF (-/-)]. Immunohistochemistry and radioligand binding studies with subsequent autoradiography were performed. Monoclonal antibodies [SRIF, protein kinase C (rod bipolar cells marker), microtubule associated protein 1A (ganglion cell marker)] and polyclonal antibodies (anti-sst1, sst2A, sst4 receptor) were applied to 10-14 μm sections of retinas fixed in paraformaldehyde. NADPH-diaphorase reactivity was assessed histochemically. [125I]LTT SRIF-28 alone or in ..