Enrichment of neonatal rat cardiomyocytes in primary culture facilitates long-term maintenance of contractility in vitro
Phong D Nguyen, Sarah T Hsiao, Priyadharshini Sivakumaran, Shiang Y Lim, Rodney J Dilley
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | AMER PHYSIOLOGICAL SOC | Published : 2012
Long-term culture of primary neonatal rat cardiomyocytes is limited by the loss of spontaneous contractile phenotype within weeks in culture. This may be due to loss of contractile cardiomyocytes from the culture or overgrowth of the non-cardiomyocyte population. Using the mitochondria specific fluorescent dye, tetramethylrhodamine methyl ester perchlorate (TMRM), we showed that neonatal rat cardiomyocytes enriched by fluorescence-activated cell sorting can be maintained as contractile cultures for long periods (24-wk culture vs. 2 wk for unsorted cardiomyocytes). Long-term culture of this purified cardiomyocyte (TMRM high) population retained the expression of cardiomyocyte markers, continu..View full abstract
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Awarded by National Health and Medical Research Council of Australia
This research was supported by National Health and Medical Research Council of Australia (509274, 1024817), National Heart Foundation, LEW Carty Foundation, and an Australian Postgraduate Award to P. D. Nguyen.