Journal article
Gastric sonic hedgehog acts as a macrophage chemoattractant during the immune response to Helicobacter pylori
MA Schumacher, JM Donnelly, AC Engevik, C Xiao, L Yang, S Kenny, A Varro, F Hollande, LC Samuelson, Y Zavros
Gastroenterology | W B SAUNDERS CO-ELSEVIER INC | Published : 2012
Abstract
Background & Aims: Macrophages mediate the epithelial response to Helicobacter pylori and are involved in the development of gastritis. Sonic Hedgehog (Shh) regulates gastric epithelial differentiation and function, but little is known about its immunoregulatory role in the stomach. We investigated whether gastric Shh acts as a macrophage chemoattractant during the innate immune response to H pylori infection. Methods: Mice with parietal cell-specific deletion of Shh (PC-ShhKO) and control mice were infected with H pylori. Levels of gastric Shh, cytokines, and chemokines were assayed by quantitative reverse-transcriptase polymerase chain reaction or by a Luminex-based multiplex assay 2, 7, o..
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Awarded by National Institutes of Health
Funding Acknowledgements
Supported by National Institutes of Health grant 1R01DK083402-01 (to Y.Z.) and in part by the Digestive Health Center of Cincinnati Children's Medical Health Center (DHC: Bench to Bedside Research in Pediatric Digestive Disease) grant CHTF/SUB DK078392. Monica DeLay, manager of the Research Flow Cytometry Core in the Division of Rheumatology at Cincinnati Children's Hospital Medical Center, provided assistance and was supported in part by National Institutes of Health grant AR-47363. All flow cytometric data were acquired using equipment maintained by the Research Flow Cytometry Core in the Division of Rheumatology at Cincinnati Children's Hospital Medical Center, which was supported in part by National Institutes of Health grant AR-47363.