Journal article
Hedgehog signaling regulates E-cadherin expression for the maintenance of the actin cytoskeleton and tight junctions
C Xiao, SA Ogle, MA Schumacher, N Schilling, RA Tokhunts, MA Orr-Asman, ML Miller, DJ Robbins, F Hollande, Y Zavros
American Journal of Physiology Gastrointestinal and Liver Physiology | Published : 2010
Abstract
In the stomach, strictly regulated cell adherens junctions are crucial in determining epithelial cell differentiation. Sonic Hedgehog (Shh) regulates epithelial cell differentiation in the adult stomach. We sought to identify whether Shh plays a role in regulating adherens junction protein E-cadherin as a mechanism for epithelial cell differentiation. Mouse nontumorigenic gastric epithelial (IMGE-5) cells treated with Hedgehog signaling inhibitor cyclopamine and anti-Shh 5E1 antibody or transduced with short hairpin RNA against Skinny Hedgehog (IMGE-5Ski) were cultured. A mouse model expressing a parietal cell-specific deletion of Shh (HKCre/ShhKO) was used to identify further changes in adh..
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Awarded by National Institute of Diabetes and Digestive and Kidney Diseases
Funding Acknowledgements
We acknowledge the assistance Monica DeLay, manager of the Research Flow Cytometry Core in the Division of Rheumatology at Cincinnati Children's Hospital Medical Center, supported in part by National Institutes of Health (NIH) AR-47363. All flow cytometric data were acquired by using equipment maintained by the Research Flow Cytometry Core in the Division of Rheumatology at Cincinnati Children's Hospital Medical Center, supported in part by NIH AR-47363.This work was supported by the American Cancer Society Research Scholar Award 119072-RSG-10-167-01-MPC (Y. Zavros) and in part by the Digestive Health Center Cincinnati Children's Medical Health Center (DHC: Bench to Bedside Research in Pediatric Digestive Disease) Pilot and Feasibility Project award CHTF/SUB DK078392 (Y. Zavros).