Journal article
Research resource: Nuclear receptors as transcriptome: Discriminant and prognostic value in breast cancer
GEO Muscat, NA Eriksson, K Byth, S Loi, D Graham, S Jindal, MJ Davis, C Clyne, JW Funder, ER Simpson, MA Ragan, E Kuczek, PJ Fuller, WD Tilley, PJ Leedman, CL Clarke
Molecular Endocrinology | Published : 2013
DOI: 10.1210/me.2012-1265
Abstract
To identify biologically relevant groupings or clusters of nuclear receptors (NR) that are associated with breast neoplasia, with potentially diagnostic, discriminant or prognostic value, we quantitated mRNA expression levels of all 48 members of the human NR superfamily by TaqMan low-density array analysis in 116 curated breast tissue samples, including pre- and postmenopausal normal breast and both ERα+ and ERα- tumor tissue. In addition, we have determined NR levels in independent cohorts of tamoxifen-treated ERα+ and ERα- tissue samples. There were differences in relative NR mRNA expression between neoplastic and normal breast, and between ER+ and ER- tumors. First, there is overexpressi..
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Funding Acknowledgements
This work was supported by a Collaborative Program Grant from the National Breast Cancer Foundation Australia. G.E.O.M. was a University of Queensland Vice Chancellors Research Fellow, E.R.S., P.J.F., and C.L.C. were research fellows of the National Health and Medical Research Council of Australia. Breast cancer and normal tissues were provided by Australian Breast Cancer Tissue Bank (ABCTB), which is supported by the National Health and Medical Research Council of Australia, the Cancer Institute NSW, and the National Breast Cancer Foundation; or by the Victorian Cancer BioBank Australia, which is supported by the Victorian Government. ABCTB tissues and samples were made available to researchers on a nonexclusive basis. Normal breast biopsies were obtained from the Susan G. Komen for the Cure Tissue Bank at the IU Simon Cancer Center. We thank contributors to the Susan G. Komen for the Cure Tissue Bank, including Indiana University, which collected the samples used in this study, as well as parents and families whose participation and help made this work possible.