Hypoxic Conditioning Enhances the Angiogenic Paracrine Activity of Human Adipose-Derived Stem Cells
Sarah T Hsiao, Zerina Lokmic, Hitesh Peshavariya, Keren M Abberton, Gregory J Dusting, Shiang Y Lim, Rodney J Dilley
STEM CELLS AND DEVELOPMENT | MARY ANN LIEBERT, INC | Published : 2013
Human adipose-derived stem cells (ASCs) secrete cytokines and growth factors that can be harnessed in a paracrine fashion for promotion of angiogenesis, cell survival, and activation of endogenous stem cells. We recently showed that hypoxia is a powerful stimulus for an angiogenic activity from ASCs in vitro and here we investigate the biological significance of this paracrine activity in an in vivo angiogenesis model. A single in vitro exposure of ASCs to severe hypoxia (<0.1% O2) significantly increased both the transcriptional and translational level of the vascular endothelial growth factor-A (VEGF-A) and angiogenin (ANG). The angiogenicity of the ASC-conditioned medium (ASC(CM)) was ass..View full abstract
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Awarded by National Health and Medical Research Council of Australia
The authors would like to acknowledge Priyadharshini Sivakumaran for technical assistance and grant support from the National Health and Medical Research Council of Australia (509271, 1024817), Postgraduate Scholarship (to S. H.) and Principal Research Fellowship (to G.J.D.) and support by the JO and JR Wicking Trust. The O'Brien Institute and the Centre for Eye Research Australia acknowledge the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program.