Journal article

The CSF-1 receptor fashions the intestinal stem cell niche

Dilara Akcora, Duy Huynh, Sally Lightowler, Markus Germann, Sylvie Robine, Jan R de May, Jeffrey W Pollard, E Richard Stanley, Jordane Malaterre, Robert G Ramsay

STEM CELL RESEARCH | ELSEVIER SCIENCE BV | Published : 2013

Abstract

Gastrointestinal (GI) homeostasis requires the action of multiple pathways. There is some controversy regarding whether small intestine (SI) Paneth cells (PCs) play a central role in orchestrating crypt architecture and their relationship with Lgr5+ve stem cells. Nevertheless, we previously showed that germline CSF-1 receptor (Csf1r) knock out (KO) or Csf1 mutation is associated with an absence of mature PC, reduced crypt proliferation and lowered stem cell gene, Lgr5 expression. Here we show the additional loss of CD24, Bmi1 and Olfm4 expression in the KO crypts and a high resolution 3D localization of CSF-1R mainly to PC. The induction of GI-specific Csf1r deletion in young adult mice also..

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Grants

Awarded by NIH


Awarded by Einstein Cancer Center


Awarded by NHMRC


Awarded by Cancer Council of Victoria, Australia


Awarded by Swiss National Science Foundation


Awarded by NATIONAL CANCER INSTITUTE


Awarded by Medical Research Council


Funding Acknowledgements

We thank Dr Silvia Fre for the helpful discussions. Expert microscopy assistance was provided by Dr Sarah Ellis and Mr Stephen Asquith (PMCC). Funding sources: ERS receives support from NIH #CA32551 and Einstein Cancer Center grant #5P30-CA13330. RGR and JM receive support from a NHMRC Program #487922 and Cancer Council of Victoria project grant #400217, Australia. MG is supported by a fellowship from the Swiss National Science Foundation #PBBEPS-139392.