Journal article
Efficient Measurement of Opsonising Antibodies to Plasmodium falciparum Merozoites
DL Hill, EM Eriksson, AB Carmagnac, DW Wilson, AF Cowman, DS Hansen, L Schofield
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2012
Abstract
Background: Antibodies targeting merozoites are important in protection from malaria. Therefore, merozoite surface proteins are attractive vaccine candidates. There is a need for robust functional assays to investigate mechanisms of acquired immunity and vaccine efficacy. To date, the study of merozoite phagocytosis has been confounded by the complexity and variability of in vitro assays. Methodology/Principal findings: We have developed a new flow cytometry-based merozoite phagocytosis assay. An optimized merozoite preparation technique produced high yields of merozoites separated from haemozoin. Phagocytosis by the undifferentiated THP-1 monocytic cell line was mediated only by Fc Receptor..
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Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. This work was supported by Australian National Health and Medical Research Council grant 1031212 and United States National Institutes of Health AI089686. AFC and LS are fellows of the NHMRC. DWW is supported by a Peter Doherty Australian Biomedical Fellowship from the NHMRC (APP1035715). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.