Journal article
Variability of Inducible Expression across the Hematopoietic System of Tetracycline Transactivator Transgenic Mice
M Takiguchi, LE Dow, JE Prier, CL Carmichael, BT Kile, SJ Turner, SW Lowe, DCS Huang, RA Dickins
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2013
Abstract
The tetracycline (tet)-regulated expression system allows for the inducible overexpression of protein-coding genes, or inducible gene knockdown based on expression of short hairpin RNAs (shRNAs). The system is widely used in mice, however it requires robust expression of a tet transactivator protein (tTA or rtTA) in the cell type of interest. Here we used an in vivo tet-regulated fluorescent reporter approach to characterise inducible gene/shRNA expression across a range of hematopoietic cell types of several commonly used transgenic tet transactivator mouse strains. We find that even in strains where the tet transactivator is expressed from a nominally ubiquitous promoter, the efficiency of..
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Awarded by National Cancer Institute
Funding Acknowledgements
This work was supported by the National Health and Medical Research Council of Australia (Project grants 575535 and 1024599 and Fellowship 509693 to RAD), Australian Government National Health and Medical Research Council Independent Research Institutes Infrastructure Support Scheme, the Sylvia and Charles Viertel Charitable Foundation (Fellowships to BTK and RAD), Victorian State Government OIS grants, Australian Research Council Future Fellowship (awarded to SJT) and the Victorian Endowment for Science, Knowledge and Innovation (VESKI Fellowship to RAD). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.