Molecular-based investigation of Cryptosporidium and Giardia from animals in water catchments in southeastern Australia
Matthew J Nolan, Aaron R Jex, Anson V Koehler, Shane R Haydon, Melita A Stevens, Robin B Gasser
WATER RESEARCH | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2013
There has been no large-scale systematic molecular epidemiological investigation of the waterborne protozoans, Cryptosporidium or Giardia, in southeastern Australia. Here, we explored, for the first time, the genetic composition of these genera in faecal samples from animals in nine Melbourne Water reservoir areas, collected over a period of two-years. We employed PCR-based single-strand conformation polymorphism (SSCP) and phylogenetic analyses of loci (pSSU and pgp60) in the small subunit (SSU) of ribosomal RNA and 60-kDa glycoprotein (gp60) genes to detect and characterise Cryptosporidium, and another locus (ptpi) in the triose-phosphate isomerase (tpi) gene to identify and characterise G..View full abstract
Funding support from the Australian Research Council and Melbourne Water Corporation (to RBG and ARJ) is gratefully acknowledged. Thanks to Drs Kathy Cinque and Khyrul Islam as well as Aradhana Pangasa for technical assistance. Thanks also to Dr Cinzia Cantacessi for critical comments on the draft manuscript.