Journal article
Spatial association with PTEX complexes defines regions for effector export into Plasmodium falciparum-infected erythrocytes
DT Riglar, KL Rogers, E Hanssen, L Turnbull, HE Bullen, SC Charnaud, J Przyborski, PR Gilson, CB Whitchurch, BS Crabb, J Baum, AF Cowman
Nature Communications | Published : 2013
DOI: 10.1038/ncomms2449
Abstract
Export of proteins into the infected erythrocyte is critical for malaria parasite survival. The majority of effector proteins are thought to export via a proteinaceous translocon, resident in the parasitophorous vacuole membrane surrounding the parasite. Identification of the Plasmodium translocon of exported proteins and its biochemical association with exported proteins suggests it performs this role. Direct evidence for this, however, is lacking. Here using viable purified Plasmodium falciparum merozoites and three-dimensional structured illumination microscopy, we investigate remodelling events immediately following parasite invasion.We show that multiple complexes of the Plasmodium tran..
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Funding Acknowledgements
We thank Robin Anders, Jana McBride and David Cavanagh for antibodies and Justin Boddey and Melanie Rug for input into experimental design. Erythrocytes were kindly provided by the Red Cross Blood Bank (Melbourne). This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. This work was supported by National Health and Medical Research Council of Australia. DTR is supported by a Pratt Foundation PhD scholarship; LT is supported by a Chancellor's Postdoctoral Fellowship from the University of Technology, Sydney; CBW is supported by a Senior Research Fellowship from the NHMRC; JB is supported by an ARC Future Fellowship; AFC is an Australia Fellow of the NHMRC. The funders had no role in study design, data collection and analysis, decision to publish or preparation of manuscript.