Journal article

Oligomers, fact or artefact? SDS-PAGE induces dimerization of beta-amyloid in human brain samples

Andrew D Watt, Keyla A Perez, Alan Rembach, Nicki A Sherrat, Lin Wai Hung, Timothy Johanssen, Catriona A McLean, Woan Mei Kok, Craig A Hutton, Michelle Fodero-Tavoletti, Colin L Masters, Victor L Villemagne, Kevin J Barnham

ACTA NEUROPATHOLOGICA | SPRINGER | Published : 2013

Abstract

The formation of low-order oligomers of β-amyloid (Aβ) within the brain is widely believed to be a central component of Alzheimer's disease (AD) pathogenesis. However, despite advances in high-throughput and high-resolution techniques such as xMAP and mass spectrometry (MS), investigations into these oligomeric species have remained reliant on low-resolution Western blots and enzyme-linked immunosorbent assays. The current investigation compared Aβ profiles within human cortical tissue using sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE), xMAP and surface enhanced laser desorption/ionization time-of-flight MS and found that whilst there was significant correlation ac..

View full abstract