Journal article

Mitochondrial DNA Haplotypes Define Gene Expression Patterns in Pluripotent and Differentiating Embryonic Stem Cells

Richard DW Kelly, Andrew E Rodda, Adam Dickinson, Arsalan Mahmud, Christian M Nefzger, William Lee, John S Forsythe, Jose M Polo, Ian A Trounce, Matthew McKenzie, David R Nisbet, Justin C St John



Mitochondrial DNA haplotypes are associated with various phenotypes, such as altered susceptibility to disease, environmental adaptations, and aging. Accumulating evidence suggests that mitochondrial DNA is essential for cell differentiation and the cell phenotype. However, the effects of different mitochondrial DNA haplotypes on differentiation and development remain to be determined. Using embryonic stem cell lines possessing the same Mus musculus chromosomes but harboring one of Mus musculus, Mus spretus, or Mus terricolor mitochondrial DNA haplotypes, we have determined the effects of different mitochondrial DNA haplotypes on chromosomal gene expression, differentiation, and mitochondria..

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Funding Acknowledgements

This work was supported by the Victorian Government's Operational Infrastructure Support Program; Monash Institute of Medical Research start-up funds to J.C.St.J.; an NHMRC CDA Fellowship and the James and Vera Lawson Trust to M. McK.; a Monash Larkins Program and an NMHRC CDF to J.M.P.