Journal article

Preparation of an adipogenic hydrogel from subcutaneous adipose tissue

Christopher J Poon, Maria V Pereira E Cotta, Shiba Sinha, Jason A Palmer, Alan A Woods, Wayne A Morrison, Keren M Abberton

ACTA BIOMATERIALIA | ELSEVIER SCI LTD | Published : 2013

Abstract

The ability to generate controlled amounts of adipose tissue would greatly ease the burden on hospitals for reconstructive surgery. We have previously shown that a tissue engineering chamber containing a vascular pedicle was capable of forming new fat; however, further refinements are required to enhance fat formation. The development and maintenance of engineered adipose tissue requires a suitable source of growth factors and a suitable scaffold. A hydrogel derived from adipose tissue may fulfil this need. Subcutaneous fat was processed into a thermosensitive hydrogel we refer to as adipose-derived matrix (ADM). Protein analysis revealed high levels of basement membrane proteins, collagens ..

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