Preparation of an adipogenic hydrogel from subcutaneous adipose tissue
Christopher J Poon, Maria V Pereira E Cotta, Shiba Sinha, Jason A Palmer, Alan A Woods, Wayne A Morrison, Keren M Abberton
ACTA BIOMATERIALIA | ELSEVIER SCI LTD | Published : 2013
The ability to generate controlled amounts of adipose tissue would greatly ease the burden on hospitals for reconstructive surgery. We have previously shown that a tissue engineering chamber containing a vascular pedicle was capable of forming new fat; however, further refinements are required to enhance fat formation. The development and maintenance of engineered adipose tissue requires a suitable source of growth factors and a suitable scaffold. A hydrogel derived from adipose tissue may fulfil this need. Subcutaneous fat was processed into a thermosensitive hydrogel we refer to as adipose-derived matrix (ADM). Protein analysis revealed high levels of basement membrane proteins, collagens ..View full abstract
Awarded by NBCF novel concept grant
We wish to thank Dr. Caroline Taylor, Dr. Michael Findlay, Dr. Peter Mountford and Associate Professor Anthony Penington for their input and advice and the staff at EMSU for animal surgery. The O'Brien Institute acknowledges the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program. This work was supported by the NBCF novel concept grant NC-11-01 and the DBI VSA grant Neopec.