Journal article
Convergent differentiation: Myeloid and lymphoid pathways to murine plasmacytoid dendritic cells
P Sathe, D Vremec, L Wu, L Corcoran, K Shortman
Blood | Published : 2013
Abstract
The developmental origin of IFN-producing plasmacytoid dendritic cells (pDCs) has been uncertain. In the present study, we tracked the development of pDCs in cultures of BM precursors stimulated with Flt3 ligand. Common myeloid precursors (CMPs) produced both conventional DCs (cDCs) and pDCs via the DC-restricted common DC precursor. Common lymphoid precursors (CLPs) produced only a few cDCs with variable efficiency, but produced pDCs via a transient intermediate precursor with B-cell potential. The pDCs of both origins produced IFN-α when stimulated with CpG oligonucleotides. The pDCs of CLP origin showed evidence of past RAG1 expression and had D-J rearrangements in IgH genes. Most pDCs an..
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Funding Acknowledgements
This work was supported by the National Health and Medical Research Council, Australia, and the University of Melbourne and was made possible through the Victorian State Government Operational Infrastructure Support and Australian Government (National Health and Medical Research Council of Australia Independent Research Institutes Infrastructure Support Scheme).