Journal article
Small macrophages are present in early childhood respiratory disease
LW Garratt, AKA Wright, SC Ranganathan, J Grigg, PD Sly, T Douglas, C Gangell, G Hall, A Kicic, L Mott, B Skoric
Journal of Cystic Fibrosis | ELSEVIER SCIENCE BV | Published : 2012
Abstract
Background: Recently, an established "small macrophage" phenotype has been observed in the sputum of patients with CF and COPD. However, little is known about the prevalence of this phenotype in the airways of young children. Since respiratory inflammation begins early in CF, we hypothesised that these small macrophages would be increased in paediatric CF bronchoalveolar lavage (BAL). Methods: Macrophage populations in CF and disease control BAL were assessed by multicolour flow cytometry. BAL inflammatory indices were collected as part of the AREST-CF programme. Results: Small macrophages were present in CF (n = 35, mean 36 ± 12% of BAL macrophages) but not significantly different to the re..
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Awarded by Cystic Fibrosis Foundation Therapeutics
Funding Acknowledgements
We thank Dr Siobhain Mulrennan at Sir Charles Gairdner Hospital, Western Australia for her recruitment of adult subjects. The AREST CF would like to thank the contributions of Princess Margaret Hospital for Children, The Telethon Institute for Child Health Research, Royal Children's Hospital Melbourne and Murdoch Children's Research Institute. We would also like to thank the participants and their families who contribute to the AREST CF program. We also acknowledge the support of the Cystic Fibrosis Foundation, Australia. This study is supported by Cystic Fibrosis Foundation Therapeutics (SLY04A0, STICK09A0) and the National Health and Medical Research Council (513730).