Journal article

Tip-Enhanced Raman Scattering (TERS) from Hemozoin Crystals within a Sectioned Erythrocyte

Bayden R Wood, Elena Bailo, Mehdi Asghari Khiavi, Leann Tilley, Samantha Deed, Tanja Deckert-Gaudig, Don McNaughton, Volker Deckert

Nano Letters | AMER CHEMICAL SOC | Published : 2011


Tip-enhanced Raman scattering (TERS) is a powerful technique to obtain molecular information on a nanometer scale, however, the technique has been limited to cell surfaces, viruses, and isolated molecules. Here we show that TERS can be used to probe hemozoin crystals at less than 20 nm spatial resolution in the digestive vacuole of a sectioned malaria parasite-infected cell. The TERS spectra clearly show characteristic bands of hemozoin that can be correlated to a precise position on the crystal by comparison with the corresponding atomic force microscopy (AFM) image. These are the first recorded AFM images of hemozoin crystals inside malaria-infected cells and clearly show the hemozoin crys..

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Awarded by BMBF

Funding Acknowledgements

We would like to acknowledge Professor Yukihiro Ozaki, Dr. Tamitake Itoh, and Dr. Kazuhiro Hashimoto, (Department of Chemistry, Kwansei Gakuin University, Sanda, Japan) for assistance in recording the SERS spectrum of beta-hematin and Dr. Christian Budich for assistance with TERS measurements and instrumentation. We also thank the Alexander von Humboldt Foundation and the BMBF ("Nanotechnologie" Grant 0312032C) and the Australian Research Council for financial support.