Journal article
Discriminating the intraerythrocytic lifecycle stages of the malaria parasite using synchrotron FT-IR microspectroscopy and an artificial neural network
GT Webster, KA De Villiers, TJ Egan, S Deed, L Tilley, MJ Tobin, KR Bambery, D McNaughton, BR Wood
Analytical Chemistry | AMER CHEMICAL SOC | Published : 2009
DOI: 10.1021/ac802291a
Abstract
Synchrotron Fourier transform infrared (FT-IR) spectra of fixed single erythrocytes infected with Plasmodium falciparum at different stages of the intraerythrocytic cycle are presented for the first time. Bands assigned to the hemozoin moiety at 1712, 1664, and 1209 cm-1 are observed in FT-IR difference spectra between uninfected erythrocytes and infected trophozoites. These bands are also found to be important contributors in separating the trophozoite spectra from the uninfected cell spectra in principal components analysis. All stages of the intraerythrocytic lifecycle of the malarial parasite, including the ring and schizont stage, can be differentiated by visual inspection of the C-H st..
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Awarded by National Research Foundation
Funding Acknowledgements
This work is supported by an Australian Research Council Discovery Grant. Dr. Bayden Wood was supported by an Australian Synchrotron Research Fellow Grant and a Monash Synchrotron Research Fellowship. We thank the IR beamline scientist Dr. Ljiljana Puskar from the Australian Synchrotron for FT-IR beam-time and experimental support. Katherine de Villiers was supported by an Equity Development Program scholarship of the Department of Chemistry, University of Cape Town. Support (Timothy Egan and Katherine de Villiers) also came from the National Research Foundation under Grant No. 2061833 and the Medical Research Council of South Africa.