Journal article
Demonstration of islet-autoreactive CD8 T cells in insulitic lesions from recent onset and long-term type 1 diabetes patients
KT Coppieters, F Dotta, N Amirian, PD Campbell, TWH Kay, MA Atkinson, BO Roep, MG von Herrath
Journal of Experimental Medicine | Published : 2012
DOI: 10.1084/jem.20111187
Abstract
A direct association of islet-autoreactive T cells with β cell destruction in human pancreatic islets from type 1 diabetes (T1D) patients has never been demonstrated, and little is known about disease progression after diagnosis. Frozen pancreas samples were obtained from 45 cadaveric T1D donors with disease durations ranging from 1 wk to >50 yr, 14 nondiabetic controls, 5 nondiabetics with islet autoantibodies, 2 cases of gestational diabetes, and 6 T2D patients. Sections were systematically analyzed for the presence of insulin-sufficient β cells, CD8+ insulitic lesions, and HLA class I hyperexpression. Finally, consecutive sections from HLA-A2-expressing individuals were probed for CD8 T c..
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Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
This research was performed with the support of the nPOD, a collaborative T1D research project sponsored by the Juvenile Diabetes Research Foundation International (JDRF). Organ Procurement Organizations, partnering with nPOD to provide research resources, are listed at www.jdrfnpod.org/our-partners.php. This work was supported by a National Institutes of Allergy and Infectious Diseases U-prevention center grant to M. G. von Herrath and the Brehm Coalition. F. Dotta is supported by the European Union (Collaborative Project NAIMIT in the Framework Program 7), by the Italian Ministry of Research, by the Italian Ministry of Health, and by the Tuscany Region.