Journal article
Method of bacterial killing differentially affects the human innate immune response to Staphylococcus epidermidis
T Strunk, P Richmond, A Prosser, K Simmer, O Levy, D Burgner, A Currie
Innate Immunity | SAGE PUBLICATIONS LTD | Published : 2011
Abstract
Background:In vitro investigations of human innate immune responses to extracellular bacteria commonly utilise killed preparations in preference to live organisms. The effects of the bacterial preparation method on the activation of innate signalling pathways by the common opportunistic pathogen Staphylococcus epidermidis (SE) are unknown.Materials and methods: Mononuclear cell cytokine expression patterns induced by live (LSE), heat-killed (HKSE) and ethanol-killed SE (EKSE) were characterized at the transcriptional and translational level. Toll-like receptor (TLR)-activating capacity of the preparations was analysed using TLR-transfected human embryonic kidney cells.Results: Live SE activa..
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Awarded by National Institutes of Health
Funding Acknowledgements
This study was supported by Princess Margaret Hospital, Women's and Infants' Research Foundation (WA), Clive and Vera Ramaciotti Medical Research Foundation, University of Western Australia, Rebecca Cooper Medical Research Foundation, Channel 7 Telethon, European Society for Paediatric Infectious Diseases and the National Health and Medical Research Council, Australia (Project grant 513847). Dr Strunk was supported by a Research Fellowship of the Deutsche Forschungsgemeinschaft (STR1022/1-1) and by an International Postgraduate Research Scholarship of the University of Western Australia. Dr Ofer Levy's laboratory is supported by National Institutes of Health (NIAID) RO1 AI067353-01A1 and Dr Levy is a recipient of a Harvard-Australia Fellowship. Dr Andrew Currie is supported by a fellowship from the BrightSpark Foundation, WA.