Activation tagging systems in rice

Abstract

Sequencing of the 389-Mb rice genome (Oryza sativa L.) is nearly complete and map-based, finished quality sequence now covers 95% of the genome. Determining function of the 37,544 predicted genes in the rice genome, however, remains a formidable challenge that will require multiple, Despite widespread application, the traditional knockout approach is limited in its ability to fully saturate the rice genome with mutations. Genes with lethal or deleterious knockout phenotypes (particularly at the embryonic stage of development) are not amenable to the loss-offunction approach, and the investigation of large gene families is often hampered by the redundant activity of one gene member compensati..