High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography
Sebastien Boutet, Lukas Lomb, Garth J Williams, Thomas RM Barends, Andrew Aquila, R Bruce Doak, Uwe Weierstall, Daniel P DePonte, Jan Steinbrener, Robert L Shoeman, Marc Messerschmidt, Anton Barty, Thomas A White, Stephan Kassemeyer, Richard A Kirian, M Marvin Seibert, Paul A Montanez, Chris Kenney, Ryan Herbst, Philip Hart Show all
Science | AMER ASSOC ADVANCEMENT SCIENCE | Published : 2012
Awarded by U.S. NSF
Awarded by NIH
Awarded by German Federal Ministry for Education and Research
Awarded by Deutsche Forschungsgemeinschaft Cluster of Excellence
Portions of this research were carried out at the LCLS, a National User Facility operated by Stanford University on behalf of the U.S. Department of Energy (DOE), Office of Basic Energy Sciences (OBES) and at the Swiss Light Source, beamline X10SA, Paul Scherrer Institute, Villigen, Switzerland. The CXI instrument was funded by the LCLS Ultrafast Science Instruments (LUSI) project funded by DOE, OBES. We acknowledge support from the Max Planck Society, the Hamburg Ministry of Science and Research, and the Joachim Herz Stiftung, as part of the Hamburg Initiative for Excellence in Research (LEXI); the Hamburg School for Structure and Dynamics in Infection; the U.S. NSF (awards 0417142 and MCB-1021557); the NIH (award 1R01GM095583); the German Federal Ministry for Education and Research (grants 01KX0806 and 01KX0807); the Deutsche Forschungsgemeinschaft Cluster of Excellence EXC 306; AMOS program within the Chemical Sciences, Geosciences, and Biosciences Division of the OBES, Office of Science, U.S. DOE; the Swedish Research Council; the Swedish Foundation for International Cooperation in Research and Higher Education. We thank A. Meinhart and E. Hofmann for collecting the synchrotron data set, M. Gebhart for help preparing the crystals, and M. Hayes and the technical staff of SLAC and the LCLS for their great support in carrying out these experiments. Special thanks to G. M. Stewart, T. Anderson, and SLAC Infomedia for generating Fig. 1. The structure factors and coordinates have been deposited with the Protein Data Bank (accession codes 4ET8, 4ET9, 4ETA, 4ETB, 4ETC, 4ETD, and 4ETE). The diffraction patterns have been deposited with the Coherent X-ray Imaging Data Bank cxidb.org (accession code ID-17). The Arizona Board of Regents, acting for and on behalf of Arizona State University and in conjunction with R. B. D., U. W., D. P. D., and J.C.H.S., has filed U.S. and international patent applications on the nozzle technology applied herein.