Journal article
Regulation of mitochondrial protein import by cytosolic kinases
O Schmidt, AB Harbauer, S Rao, B Eyrich, RP Zahedi, D Stojanovski, B Schönfisch, B Guiard, A Sickmann, N Pfanner, C Meisinger
Cell | Published : 2011
Abstract
Mitochondria import a large number of nuclear-encoded proteins via membrane-bound transport machineries; however, little is known about regulation of the preprotein translocases. We report that the main protein entry gate of mitochondria, the translocase of the outer membrane (TOM complex), is phosphorylated by cytosolic kinases - in particular, casein kinase 2 (CK2) and protein kinase A (PKA). CK2 promotes biogenesis of the TOM complex by phosphorylation of two key components, the receptor Tom22 and the import protein Mim1, which in turn are required for import of further Tom proteins. Inactivation of CK2 decreases the levels of the TOM complex and thus mitochondrial protein import. PKA pho..
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Awarded by Ministerium fur Innovation, Wissenschaft, Forschung und Technologie des Landes Nordrhein-Westfalen, Sonderforschungsbereich
Funding Acknowledgements
We thank Drs. C.V. Glover, A.P. Bidwai, K.M. Dombek, and C. Mann for strains and antisera; Drs. N. Wiedemann, K. Wagner, T. Becker, N. Gebert, M. Bohnert, M. Gebert, and D. Stroud for discussion; and C. Prinz for expert technical assistance. This work was supported by the Deutsche Forschungsgemeinschaft, Excellence Initiative of the German Federal and State Governments (EXC 294 BIOSS; GSC-4 Spemann Graduate School), Bundesministerium fur Bildung und Forschung (Dynamo), Ministerium fur Innovation, Wissenschaft, Forschung und Technologie des Landes Nordrhein-Westfalen, Sonderforschungsbereich 746, Gottfried Wilhelm Leibniz Program, and Landesforschungspreis Baden-Wurttemberg. This paper is dedicated to Klaus Paal (1955-2009), who is deeply missed.