Journal article

Development of genetically homogenised populations of the crown rust pathogen (Puccinia coronata f.sp lolii) for disease trait dissection in perennial ryegrass (Lolium perenne L.)

PM Dracatos, MP Dobrowolski, J Lamb, RS Olle, AR Gendall, NOI Cogan, KF Smith, JW Forster

Australasian Plant Pathology | SPRINGER | Published : 2009

Abstract

Genetic analysis of host resistance to the crown rust pathogen of ryegrasses has traditionally involved the use of composite urediniospore collections, which may contain multiple genotypes and virulence races. Consequently, discrimination between major gene responses to alternative races and quantitative resistance has proved challenging for disease trait-dissection studies. A method of propagation on detached leaves has been used to derive genetically homogenised crown rust pathogen lines from bulked spore collections obtained from five distinct Australian locations. Spores from individual pustules were repeatedly reinoculated onto fresh leaves. Amplicon complexity was assessed at the concl..

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Funding Acknowledgements

This work was supported by funding from the Victorian Department of Primary Industries, Dairy Australia, the Geoffrey Gardiner Dairy Foundation, Meat and Livestock Australia and the Molecular Plant Breeding Cooperative Research Centre. The authors thank Dr Charles Brummer (University of Georgia, USA) and Professor Michael Hayward (Rhydgoch Genetics, Aberystwyth, UK) for careful critical reading of the manuscript.