Journal article

Functionally associated molecular genetic marker map construction in perennial ryegrass (Lolium perenne L.)

MJ Faville, AC Vecchies, M Schreiber, MC Drayton, LJ Hughes, ES Jones, KM Guthridge, KF Smith, T Sawbridge, GC Spangenberg, GT Bryan, JW Forster

THEORETICAL AND APPLIED GENETICS | SPRINGER | Published : 2004

Abstract

A molecular marker-based map of perennial ryegrass (Lolium perenne L.) has been constructed through the use of polymorphisms associated with expressed sequence tags (ESTs). A pair-cross between genotypes from a North African ecotype and the cultivar Aurora was used to generate a two-way pseudo-testcross population. A selection of 157 cDNAs assigned to eight different functional categories associated with agronomically important biological processes was used to detect polymorphic EST-RFLP loci in the F(1)(NA(6) x AU(6)) population. A comprehensive set of EST-SSR markers was developed from the analysis of 14,767 unigenes, with 310 primer pairs showing efficient amplification and detecting 113 ..

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