Journal article
A time-calibrated multi-gene phylogeny of the diatom genus Pinnularia
C Souffreau, H Verbruggen, AP Wolfe, P Vanormelingen, PA Siver, EJ Cox, DG Mann, BVD Vijver, K Sabbe, W Vyverman
Molecular Phylogenetics and Evolution | Published : 2011
Abstract
Pinnularia is an ecologically important and species-rich genus of freshwater diatoms (Bacillariophyceae) showing considerable variation in frustule morphology. Interspecific evolutionary relationships were inferred for 36 Pinnularia taxa using a five-locus dataset. A range of fossil taxa, including newly discovered Middle Eocene forms of Pinnularia, was used to calibrate a relaxed molecular clock analysis and investigate temporal aspects of the genus' diversification. The multi-gene approach resulted in a well-resolved phylogeny of three major clades and several subclades that were frequently, but not universally, delimited by valve morphology. The genus Caloneis was not recovered as monophy..
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Awarded by National Science Foundation
Funding Acknowledgements
We thank Aloisie Poulickova for providing cultures of Pinnularia borealis and Elie Verleyen for providing live environmental samples. Alex Ball enthusiastically assisted with confocal microscopy. Sequencing was done by Andy Vierstrate. Funding was provided by the Fund for Scientific Research-Flanders (FWO-Flanders, comprising a PhD fellowship to CS, post-doctoral fellowships to HV and PV, and Research Grant 3G/0533/07). Research on fossil diatoms is partially supported by the US National Science Foundation (DEB-0716606 to PAS) and the Natural Sciences and Engineering Council of Canada (APW). Morphological analyses at the Natural History Museum, London, were supported by an EU framework 7 SYNTHESYS Grant (GB-TAF-77) to CS.