Journal article
A multi-locus time-calibrated phylogeny of the brown algae (Heterokonta, Ochrophyta, Phaeophyceae): Investigating the evolutionary nature of the "brown algal crown radiation"
T Silberfeld, JW Leigh, H Verbruggen, C Cruaud, B de Reviers, F Rousseau
Molecular Phylogenetics and Evolution | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2010
Abstract
The most conspicuous feature in previous phaeophycean phylogenies is a large polytomy known as the brown algal crown radiation (BACR). The BACR encompasses 10 out of the 17 currently recognized brown algal orders. A recent study has been able to resolve a few nodes of the BACR, suggesting that it may be a soft polytomy caused by a lack of signal in molecular markers. The present work aims to refine relationships within the BACR and investigate the nature and timeframe of the diversification in question using a dual approach. A multi-marker phylogeny of the brown algae was built from 10 mitochondrial, plastid and nuclear loci (>10,000 nt) of 72 phaeophycean taxa, resulting in trees with well-..
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Funding Acknowledgements
We acknowledge Tristan Le Goff, Alan Millar, Kathy Miller, Richard Moe, Claude Payri, Akira Peters, Nathalie Simon and Nick Yee for their kind supply of silica-gel dried brown algal samples. We thank the Computational biology service unit of the Cornell University (http://cbsu.tc.cornell.edu/) for making available online their MrBayes and BEAST cluster facilities. Thomas Silberfeld is most grateful to Michael Manuel (UPMC) for enlightening scientific discussions, Marie-Lilith Patou and Julien Lorion for their assistance with the molecular dating methods, Anna Plassart and Lucie Bittner for their undivided support. Heroen Verbruggen thanks Stefano Draisma and Steve LoDuca for sharing their insights and opinions about brown algal fossils. The molecular work was undertaken at the "Service de Systematique Moleculaire" of the Museum National d'Histoire Naturelle (UMS 2700); we are grateful to Annie Tillier, Josie Lambourdiere and Celine Bonillo for their help and advice in the molecular lab. Finally we thank two anonymous referees for their thorough revisions and constructive criticism on a first version of the manuscript. This work was supported by the "Consortium National de Recherche en Genomique"; it is part of the agreement No. 2005/67 between the Genoscope and the Museum National d'Histoire Naturelle on the project "Macrophylogeny of life" directed by Prof. Guillaume Lecointre. Thomas Silberfeld benefited of a grant from the French 'Ministere de l'enseignement superieur et de la recherche'.