Journal article
A high-affinity ErbB4Fc fusion protein is a potent antagonist of heregulin-mediated receptor activation
EJ Koziolek, JF Donoghue, JD Bentley, G Lovrecz, O Dolezal, CW Ward, J Rothacker, EC Nice, AW Burgess, M Hafner, TG Johns, TE Adams
Growth Factors | INFORMA HEALTHCARE | Published : 2012
Abstract
Ligand-mediated activation of ErbB3 and ErbB4 is implicated in the pathogenesis of several human malignancies including cancer of the ovary and melanoma. We have used the broad ErbB ligand specificity of ErbB4 to assemble and express an ErbB4 fusion protein comprising the first 497 amino acids of the mature ErbB4 ectodomain fused to the human IgG Fc constant region. The purified fusion protein, designated sErbB4.497.Fc, binds the ErbB receptor ligands betacellulin and heregulin-β1 (HRG-β1) with high affinity (K+ADw-inf+AD4-D+ADw-/inf+AD4- 130 pM), an increase in affinity of 10-to 20-fold, respectively, compared with sErbB4.615.Fc. sErbB4.497.Fc inhibited ligand-stimulated phosphorylation of ..
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Funding Acknowledgements
The work was partially funded by the Daimler and Benz Foundation, Ladenburg, Germany and Aventis Foundation, Frankfurt, Germany. Terrance Johns is a Clinical Research Fellow for the Victorian Cancer Agency. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.