Journal article

Solubilisation of the armadillo-repeat protein beta-catenin using a zwitterionic detergent allows resolution of phosphorylated forms by 2DE

Meredith J Layton, Nicole L Church, Maree C Faux, Hong Ji, Robert JA Goode, Eugene A Kapp, Antony W Burgess, Richard J Simpson

Electrophoresis | WILEY-BLACKWELL | Published : 2012

DOI: 10.1002/elps.201100671

Grants

Awarded by National Health & Medical Research Council of Australia

Awarded by RD Wright Fellowship

Funding Acknowledgements

This work was supported by the National Health & Medical Research Council of Australia for Program Grants #487922 and #280912 and RD Wright Fellowship #164821 (M.J.L).

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Keywords

Posttranslational Modification
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Wnt Signalling
Carcinogenesis
Betaine
Transcription Factor 4
Caco-2 Cells
Mice
L Cells
Molecular Sequence Data
Cell-Line
Beta Catenin
2dimensional Gel Electrophoresis
Life Sciences & Biomedicine
Expression
Protein Processing, Post-Translational
Recombinant Proteins
Science & Technology
Isoelectric Focusing
Amino Acid Sequence
Mass-Spectrometry
Tyrosine Phosphorylation
Electrophoresis, Gel, Two-Dimensional
Biochemical Research Methods
Roles
Phosphorylation
Transcription Factors
Wnt Signaling Pathway
Colorectal-Cancer
Amidosulfobetaine
Physical Sciences
Chemistry
2-Dimensional Electrophoresis
Animals
Nuclear-Localization
Chemistry, Analytical
Solubility
Biochemistry & Molecular Biology
Wnt Pathway
Humans