Journal article

Solubilisation of the armadillo-repeat protein beta-catenin using a zwitterionic detergent allows resolution of phosphorylated forms by 2DE

Meredith J Layton, Nicole L Church, Maree C Faux, Hong Ji, Robert JA Goode, Eugene A Kapp, Antony W Burgess, Richard J Simpson

Electrophoresis | WILEY-BLACKWELL | Published : 2012

DOI: 10.1002/elps.201100671

Grants

Awarded by National Health & Medical Research Council of Australia

Awarded by RD Wright Fellowship

Funding Acknowledgements

This work was supported by the National Health & Medical Research Council of Australia for Program Grants #487922 and #280912 and RD Wright Fellowship #164821 (M.J.L).

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Keywords

Posttranslational Modification
Mice
Isoelectric Focusing
Electrophoresis, Gel, Two-Dimensional
Mass-Spectrometry
Colorectal-Cancer
Chemistry
2-Dimensional Electrophoresis
Cell-Line
Wnt Pathway
Animals
Transcription Factors
Physical Sciences
Humans
Solubility
L Cells (cell Line)
Betaine
Carcinogenesis
Nuclear-Localization
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Wnt Signaling Pathway
2dimensional Gel Electrophoresis
Caco-2 Cells
Roles
Protein Processing, Post-Translational
Life Sciences & Biomedicine
Science & Technology
Expression
Beta Catenin
Recombinant Proteins
Wnt Signalling
Amidosulfobetaine
Biochemistry & Molecular Biology
Amino Acid Sequence
Transcription Factor 4
Chemistry, Analytical
Biochemical Research Methods
Phosphorylation
Molecular Sequence Data
Tyrosine Phosphorylation