Identification of a Wnt-induced protein complex by affinity proteomics using an antibody that recognizes a sub-population of beta-catenin
Meredith J Layton, Maree C Faux, Nicole L Church, Bruno Catimel, Nadia J Kershaw, Eugene A Kapp, Cameron Nowell, Janine L Coates, Antony W Burgess, Richard J Simpson
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | ELSEVIER SCIENCE BV | Published : 2012
β-catenin is a signaling protein with diverse functions in cell adhesion and Wnt signaling. Although β-catenin has been shown to participate in many protein-protein interactions, it is not clear which combinations of β-catenin-interacting proteins form discrete complexes. We have generated a novel antibody, termed 4B3, which recognizes only a small subset of total cellular β-catenin. Affinity proteomics using 4B3, in combination with subcellular fractionation, has allowed us to define a discrete trimeric complex of β-catenin, α-catenin and the tumor suppressor APC, which forms in the cytoplasm in response to Wnt signaling. Depletion of the limiting component of this complex, APC, implicates ..View full abstract
Awarded by National Health & Medical Research Council of Australia
Awarded by RD Wright Fellowship
We thank Stephen Cody and Cameron Nowell for assistance with all aspects of microscopy. This work was supported by the National Health & Medical Research Council of Australia through program grants #487922 and #280912 and the RD Wright Fellowship #164821 (M.J.L). The authors declare that they have no conflicts of interest with regard to this manuscript.