Journal article

Identification of a Wnt-induced protein complex by affinity proteomics using an antibody that recognizes a sub-population of beta-catenin

Meredith J Layton, Maree C Faux, Nicole L Church, Bruno Catimel, Nadia J Kershaw, Eugene A Kapp, Cameron Nowell, Janine L Coates, Antony W Burgess, Richard J Simpson

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | ELSEVIER SCIENCE BV | Published : 2012

Abstract

β-catenin is a signaling protein with diverse functions in cell adhesion and Wnt signaling. Although β-catenin has been shown to participate in many protein-protein interactions, it is not clear which combinations of β-catenin-interacting proteins form discrete complexes. We have generated a novel antibody, termed 4B3, which recognizes only a small subset of total cellular β-catenin. Affinity proteomics using 4B3, in combination with subcellular fractionation, has allowed us to define a discrete trimeric complex of β-catenin, α-catenin and the tumor suppressor APC, which forms in the cytoplasm in response to Wnt signaling. Depletion of the limiting component of this complex, APC, implicates ..

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