Independent trafficking of the KCNQ1 K channel and H -K -ATPase in gastric parietal cells from mice

Abstract

Gastric acid secretion by the H+-K+-ATPase at the apical surface of activated parietal cells requires luminal K+ provided by the KCNQ1/KCNE2 K+ channel. However, little is known about the trafficking and relative spatial distribution of KCNQ1 and H+-K+-ATPase in resting and activated parietal cells and the capacity of KCNQ1 to control acid secretion. Here we show that inhibition of KCNQ1 activity quickly curtails gastric acid secretion in vivo, even when the H+-K+-ATPase is permanently anchored in the apical membrane, demonstrating a key role of the K+ channel in controlling acid secretion. Threedimensional imaging analysis of isolated mouse gastric units revealed that the majority of KCNQ1 ..